Tail fibers are specialized, proteinaceous appendages found at the base of the tail sheath in many bacteriophages (viruses that infect bacteria), serving as the primary structures for host cell recognition and initial reversible attachment. They act as the virus's “grappling hooks,” initiating the infection process by facilitating DNA transfer. Tail fibers vary in **length. Tail fibers are structures on the phage that mediate their initial interaction with bacterial hosts, allowing them to recognize and attach to the bacterial surface. This initial binding is a fundamental step that dictates whether a phage can successfully infect a particular bacterial cell. Tail. Here, using a minimal myocyanophage, termed Pam3, isolated from Lake Chaohu, we demonstrate that the chaperone gp25 forms a stable complex with the tail fiber gp24 at a stoichiometry of 3:3. 1-Å cryo-electron microscopy structure of this complex revealed an elongated structure with the gp25. Capsids are constructed from protein subunits known as capsomers, which self-assemble into a precise geometric configuration. nfections around the world is causing a global health crisis. The Gram-negative bacterium Acinetobacter baumannii is categorized as a Priority 1 pathogen for research and development of new antimicrobials by the World Health Organization due to its numerous intrinsic antibiotic resistance mechanis.