Qualitative mass spectrometry focuses on identifying the chemical structure and composition of compounds in a sample. Identifying the presence of a specific molecule relies heavily on interpreting fragmentation patterns and accurate mass. This review maps the physicochemical properties of phenolic acids and flavonoids and explains their diagnostic LC–ESI–MS/MS fragmentation routes. It then details qualitative and quantitative MS analysis, covering solvent choice, control of ionization and adducts, chromatographic selectivity, matrix. What are GC/SQ, TQ, and Q-TOF and When to Use Them? example) scanned from 35 m/z to 500 m/z at 3. Qualitative analysis answers the question, "What is in this mixture?" Qualitative analysis of compounds is simple using a mass detector (MS), since the mass ion detected represents the molecular weight of the. Mass Spectrometry (MS) has become an indispensable tool in pharmaceutical analysis due to its unparalleled sensitivity, specificity, and versatility. While quantitative whole-body autoradiography and microautoradiography are the traditional techniques for molecular imaging of drug delivery and metabolism, MSI provides advantageous specificity that.